Fig. 6.

TSA treatment effects on estrogen metabolism enzyme expression in MCF10A series and MCF7cell lines. a Concentration-dependent effects of TSA treatment on SULT1E1 mRNA expression in MCF10CA1a cells. MCF10CA1a cells were treated for 24 h with 0.1% DMSO or with 75, 150 or 300 ng/ml TSA and harvested for measurement of SULT1E1 mRNA levels with a TaqMan Gene Expression assay. All values represent the mean ± SEM of three independent cell culture experiments relative to the amount measured in the DMSO-treated cells. *** Significantly different from the DMSO-treated group, P < 0.001. Inset Correlation of TSA-mediated changes in SULT1E1 enzymatic activity with changes in SULT1E1 mRNA levels. b MCF10A series and MCF7 cell lines were treated for 24 h with 0.1% DMSO or 300 ng/ml TSA and SULT1E1 mRNA levels were measured. SULT1E1 mRNA contents are expressed relative to the level measured in DMSO-treated MCF10A cells. All values represent the mean ± SEM of three independent experiments. * Significantly different from the corresponding DMSO-treated group (P < 0.05). c Cells were treated with as indicated in b and estrogen metabolism enzyme mRNA levels were measured. STS, COMT, CYP1B1, 17βHSD1, and 17βHSD2 mRNA contents are expressed relative to the respective levels measured in DMSO-treated MCF10A cells. SULT1A1 and SULT2B1 mRNA levels are expressed relative to the levels measured in DMSO-treated MCF7 cells. SULT2A1 mRNA levels are expressed relative to the level measured in TSA-treated MCF7 cells. CYP19 mRNA levels are expressed relative to the level measured in TSA-treated MCF10A cells. All values represent the mean ± SEM of three independent experiments. *, ** Significantly different from the corresponding DMSO-treated group, P < 0.05 and P < 0.01, respectively