Fig. 5.

Visualization of TAT fusion proteins transduced into IMR90 fibroblast cells by Immunofluoresence. 40 nM TAT fusion proteins were added to culture media of IMR90 cells. Four hours later, the cells were washed once with 0.2 M glycine-HCl, pH 2.2 to remove those proteins attached on the outer surface of the cells. Then cells were then examined by Immunofluorescence using specific antibodies. a Untreated, b treated with TAT-KLF4, c treated with TAT-c-MYC, d treated with TAT-OCT4 and e treated with TAT-SOX2. In all cases very little protein has translocated to the nucleus and remains bound to the endosomes in discreet packages. Scale bar: 20 μm