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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Anal Chem. 2011 Sep 19;83(20):7815–7822. doi: 10.1021/ac201498a

Figure 5.

Figure 5

HPLC analysis to detect OSCS contamination in heparin. (A) Injection of a standard of 1:1 di/tetrasaccharide mixture and analysis by HPLC. The elution time of each peak and area under the curve was taken as standard representation to measure product formation after enzymatic reaction. (B) HPLC profile of clean heparin after treatment with heparinase I. (C) Different weight percents of OSCS in heparin, obtained by spiking, were subjected to digestion with heparinase I. The AUC of di- and tetrasaccharides was measured for each after a limit digestion; results are plotted as a function of weight percent of OSCS. The control digestion yielded AUCs of 42 700 ± 700 for disaccharide (■) and 15 200 ± 80 for tetrasaccharide (●).