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. Author manuscript; available in PMC: 2014 Jun 27.
Published in final edited form as: Anal Chim Acta. 2013 May 13;785:27–33. doi: 10.1016/j.aca.2013.05.003

Fig. 4.

Fig. 4

Scaling correction for absorptivity effects. (A) MCR results without (left) and with (right) normalization show that the 50% Mox point is affected by normalization. (B) Concatenated second-derivative spectra. The visible region comprises 562–601 nm and the NIR region comprises 744–802 nm. (C) Up-weighting the NIR region where deoxygenated species have predominant intensity corrects for the normalization effect. This example shows results when the NIR region was up-scaled by a factor of 5.3. The optimal scaling factor was determined by adjusting the scaling until the 50% Mox points for unnormalized (left) and normalized (right) cases were equal and (D) concatenated second-derivative spectra with the scaling, without and with normalization.