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. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Biochemistry. 2009 Apr 7;48(13):2960–2968. doi: 10.1021/bi8021153

Figure 4.

Figure 4

Time courses of Pi production during single-stranded DNA translocation along poly(dT) monitored using the EnzChek® phosphate assay as described in Materials and Methods. A linear analysis of the time course resulted in an estimate of Vmax = (63 ± 2) [Pi]/[yISW2]/min, consistent with the estimate of (61 ± 4) [Pi]/[yISW2]/min determined from our radioactivity-based assay, and an intercept on the ordinate of (−1 ± 0.5) [Pi]/[yISW2]. This negative intercept on the ordinate is consistent with the kinetic model depicted in Scheme 4. A control experiment in which no DNA was included with the reaction is also shown. A linear analysis of this time course resulted in an estimate of Vmax = (2 ± 1) [Pi]/[yISW2]/min, consistent with the results from our radioactivity-based assay, and an intercept on the ordinate of (0.0 ± 0.1).