Skip to main content
. Author manuscript; available in PMC: 2013 Sep 13.
Published in final edited form as: Int J Radiat Biol. 2011 Jul 5;87(10):1052–1060. doi: 10.3109/09553002.2011.587860

Figure 1.

Figure 1

Figure 1

CBZ results in a radioprotective and mitigative effect on 32D cl 3 cells in clonogenic survival curves. Representative LQ (panels A, C, E, G) and SHMT (panels B, D, F, H) plot of colony formation by irradiated and quantified cells treated with CBZ in DMSO (dimethyl sulfoxide) added to culture medium 1 hour before or immediately after irradiation at A,B) 1 μM (p = 0.012 or 0.011, respectively), C,D) 10 μM (p = 0.010 or 0.019, respectively), E,F) 100 μM (p = 0.003 or 0.014, respectively), or G,H) CBZ in DMSO added to culture medium 1 hour before, immediately after, or both 1 hour before and immediately after irradiation at 10 μM (p = 0.040, 0.032 or 0.033, respectively). P values are for SHMT plots in reference to control, which was irradiation plus vehicle only, comparing Do values for protection and ñ values for mitigation. Error bars represent the standard error of the mean (SEM) for the 4 wells quantified for each irradiation dose in each experiment. Experiments were repeated in triplicate. DMSO was added to control irradiated cells and had no effect compared to irradiation alone.