Figure 2. Time-course leukocytes population profile in BAL fluid after CS exposure.

(A) Percentage of neutrophils, recently migrated monocytes and recently differentiated alveolar macrophages in BAL fluid from smoke-exposed group (▪) at 24, 48 and 72 h after CS exposure in C57BL/6J susceptible and 129S2/SvHsd non susceptible mice compared to those in the air exposed group (□) and expressed the mean ± SEM; *p<0.05; n = 8/group. (B) Leukocytes population was identified by flow cytometry analysis based on their characteristic properties shown in the forward scatter (FSC) and sideward scatter (SSC). Representative gating was set for Ly6B.2^hi on neutrophils (yellow), Ly6B.2⁺ on recently migrated monocytes (pink) and F4/80^hi on recently differentiated alveolar macrophages (blue) from BAL fluid of each strain of mice.