Figure 3. Inhibition of cerebroside C on the activities of LOX, PLC and PLD.

This was observed in roots of wheat seedlings during 4°C stress at 0, 6, 12, 24, 48, 72 h and recovering 25°C for 24 h (total 96 h). The results were expressed as the mean of three replicates (n = 3, 0.2 g fresh roots from 5–10 seedlings in each replicate). a. Activities of lipoxygenase (LOX), b. phospholipid C (PLC) and c. phospholipid D (PLD) in roots of wheat seedlings were determined individually using biochemical assays. CC+4°C, treated with 20 µg/mL CC under cold stress; CK+4°C, treated with 0.5% DMSO under cold stress; CK+25°C treated with 0.5% DMSO in growth chamber at 25°C.