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. 2013 Sep 13;8(9):e73742. doi: 10.1371/journal.pone.0073742

Table 1. Differentially expressed genes related to carbohydrate metabolism and cell wall biogenesis in the stems and roots of Valencia sweet orange (Citrus sinensis) caused by Ca. L. asiaticus infection.

Accession No. Gene description Log2 fold change
Stem Root
Starch synthesis
DN622894 ADP-glucose pyrophosphorylase large subunit 3 (APL3) 1.96
CB292132 Granule-bound starch synthase (GBSS) 1.82
Starch and sucrose degradation
CX046632 Exocellular acid invertase 1 (Exinv1) 2.43
CD575166 Neutral invertase −1.00
DN958063 Alpha-amylase (AMY1) 2.45
CF836730 Beta-amylase (BMY1/BMY7) −1.17
Minor carbohydrate metabolism
CN184547 putative Myo-inisitol oxygenase 1.91
CX046914 Raffinose synthase family protein (din10) 1.51
CX306291 Stachyose synthase −1.11
CD575394 Trehalose-6-phosphate phosphatase (TPPA) −1.31
CX636014 Trehalose-6-phosphate phosphatase, putative −1.64
CX309237 Trehalose-6-phosphate synthase 2 (TPS2) −1.21
CF831824 Trehalose-6-phosphate synthase 8 (TPS8) −1.63
CX306907 Trehalose-6-phosphate synthase 10 (TPS10) −1.83
Cell wall precursors
CK939533 UDP-glucose 4-epimerase −1.37
Cell wall proteins
CX638719 Extensin −1.41
CX076453 Extensin-like protein 1.22
CF417841 Proline-rich protein (PRP4) 1.09
CK938120 Glycine-rich RNA-binding protein 1.23
Cellulose synthesis
CF835773 Cellulose synthase-like C7 (CSLC7) −1.90
CV705580 Cellulose synthase catalytic subunit-like protein −1.72
DN618266 Cellulose synthase-like protein D4 (CSLD4) −1.44
CX045083 Cellulose synthase isolog 1.25
Cell wall modification
DN618428 BURP domain containing protein 2.01
CX665316 Polygalacturonase-like protein 1.10
CF835126 Expansin-related protein 1 (EXP1) 2.03
CK939135 Syringolide-induced protein 19-1-5 −2.67
CX296097 putative Xyloglucan endotransglycosylase (XET) −2.80
CV716643 Xyloglucan endotransglycosylase 1 (XET) −2.53

Accession No. is a unique identifier of EST sequences from several citrus species and hybrids linked to the NCBI. LFC is the ratio of the expression level in the infected samples compared to the healthy trees. The ratio is the mean of 3 replicates. The annotation is according to the latest available BLASTx search at non-redundant protein database at the NCBI. Metabolic pathway grouping is based on the gene ontology in the MapMan program (Thimm et al., 2004).