Figure 2. Human occludin C-terminus antibody immunoprecipitated a 55 kDa protein from E. histolytica.

Immunoprecipitation of T84 human colonic cells and Eh cellular lysates were done using a rabbit occludin antibody and subsequently immunoblotted with a mouse occludin antibody. Note the presence of 55“occludin-like” protein in Eh. The heavy chain (HC) is shown on the blots. IgG was used as isotype control. This experiment was repeated three times with similar results and representative blots are shown.