Table 1. Loci characteristics and discriminatory power of MLST schemes based on four (MLST₄) and eight (MLST₈) genes.

Loci	Size (bp)	Alleles	No. Polymorphic sites	% polymorphic sites	p-distancea	πb	θc	dN/dS	Tajima'sD testd	% discriminatory index (95% confidence intervals)
aroE	424	19	38	8.96	2.22%	0.021	0.026	0.147	−0.712	47.1 (40.1–54.1)
atpD	453	13	22	4.86	1.00%	0.010	0.016	0.150	−1.487	42.3 (35.6–49.0)
gmk	400	12	20	5.00	1.40%	0.014	0.016	0.105	−0.725	43.8 (37.1–50.5)
guaA	493	18	32	6.49	2.30%	0.022	0.019	0.025	0.545	70.6 (66.5–74.7)
lepA	452	10	16	3.54	1.20%	0.012	0.013	0.107	−0.351	45.5 (38.6–52.5)
sodA	450	14	21	4.67	1.40%	0.014	0.015	0.063	−0.244	69.0 (64.4–73.6)
tly	777	23	49	6.31	1.70%	0.016	0.018	0.299	−0.337	79.5 (76.8–82.3)
camp2	804	33	79	9.82	2.60%	0.026	0.025	0.295	0.066	83.7 (81.3–86.0)
MLST4	2498	–	–	–	1.89%	–	–	–	–	93.0 (91.3–94.5)
MLST8	4253	–	–	–	1.50%	–	–	–	–	94.0 (92.3–95.3)

^ap-distance determined using Kimura Two parameter nucleotide substitution model.

^bNucleotide diversity per site.

^cAverage number of nucleotide differences per site.

^dp>0.1 for all Tajima's D test results.