Table 1. Primers and annealing temperatures used for the amplification of each gene.

Gene	Primer (5′- 3′)	Annealing temperature (°C)		Product size (base pairs)
GAPDH	F:5′-TCAAGAAGGTGGGTGAAGCAGG-3′	60		350
	R:5′-TATTATGGGGGTCTGGGATGG-3′
IL-6	F:5′-CTACCCCAATTTCCAATGCT-3′	58		187
	R:5′-ACCACAGTGAGGAATGTCCA-3′
IL-17A	F:5′-GCTCCAGAAGGCCCTCAGA-3′	57		142
	R:5′-AGCTTTCCCTCCGCATTGA-3′
IL-10	F:5′-AGTGGAGCAGGTGAAGAGTG-3′	58	250
	R:5′-TTCGGAGAGAGGTACAAACG-3′
IFN-γ	F:5′-TCAAGTGGCATAGATGTGGAAGAA-3′	60	92
	R:5′-TGGCTCTGCAGGATTTTCATG-3′
Foxp3	F:5′-TCTCCAGGTTGCTCAAAGTC-3′	58	203
	R:5′-GCAGAAGTTGCTGCTTTAGG-3′
HP-Ura	F:5′-AAACCGGATGATGTGATG-3′	55	350
	R:5′-GGTATGCACGGTTACGAG-3′
HP 16s	F:5′-CAAGTCATGGCCCTTAC-3′	58	161
	R:5′-TTGCGATTACTAGCGATTCC-3′