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. 2013 Sep 13;8(9):e74407. doi: 10.1371/journal.pone.0074407

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© 2013 Peng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, C) Highly metastatic HCCLM3 cell line stably expressing GFP-LC3 reporter gene (HCCLM3-GFP-LC3) was generated via lentivirus-mediated GFP-LC3 overexpression. The HCCLM3-GFP-LC3 cells exhibited a large number of GFP-LC3 dots after rapamycin treatment (+Rapa). (B, C) HCCLM3 stably expressing GFP (HCCLM3-GFP) served as Control. The HCCLM3-GFP cells displayed no GFP-LC3 dots after rapamycin treatment (+Rapa). (D) Western blot analysis of LC3 showed that rapamycin induced intense autophagy in both HCCLM3-GFP-LC3 and HCCLM3-GFP.