Table 1. Results of MQAE assay.
| Preparation | F | F0 | F0/F | [Cl-]i (mmol/L) |
|---|---|---|---|---|
| NOD+Nol | 14.25±2.68 | 20.48±3.82 | 1.44 | 8.58±0.89 |
| NOD+Nel | 14.32±2.56 | 20.32±2.95 | 1.42 | 8.34±1.08 |
| NOD+SO | 18.48±2.13 | 22.38±2.87 | 1.21 | 4.11±0.68 |
| OD+Nol | 10.86±2.38 | 19.21±3.68 | 1.77 | 15.29±1.56 |
| OD+Nel | 11.30±2.32 | 20.20±4.30 | 1.78 | 15.47±1.51 |
| OD+SO | 7.21±1.52 | 18.95±3.34 | 2.63 | 32.24±2.06 |
MQAE: N-(6-methoxyquinolyl) acetoethyl ester; F: corresponding MQAE fluorescence; F0: MQAE fluorescence with ionophore and zero bath chloride; Ksv, Stern–Volmer constant; [Cl-]i: intracellular chloride concentration calculated from the Stern–Volmer equation ([Cl-]i =(F0/F-1)/ Ksv; K= 0.051 mmol-1); NOD: non-oxygen deprivation; OD: oxygen deprivation; NoI: non-infection group; NeI: negative infection group; SO: SPAK overexpression group.