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. 2013 Sep 13;8(9):e75753. doi: 10.1371/journal.pone.0075753

Figure 2. IPC increases mitochondrial SIRT1 protein levels specifically in neurons.

Figure 2

Primary cortical glial and neuronal-only cultures were exposed to oxygen and glucose deprivation for 45 min (IPC) and the level of mitochondrial SIRT1 was determined 2 and 48 hrs later (n = 6) (A). Western blot quantitation is shown in (B). SIRT1-specific deacetylase activity was determined in mitochondrial lysates from neuronal cultures 48 hrs following exposure to sham or IPC (n = 4) (C). Neuronal mitochondria acetylation levels were determined at 2 and 48 hrs following IPC exposure (n =3) (D). * p < 0.05 increase from sham by one-way analysis of variance (ANOVA) and Dunnetts’ multiple comparison post hoc test.