Skip to main content
. 2013 Sep 13;8(9):e75753. doi: 10.1371/journal.pone.0075753

Figure 4. Epsilon PKC regulates mitochondrial SIRT1 protein levels.

Figure 4

Neuronal cultures were treated with the PKCε activator ψεRACK (100 nM) or with the carrier TAT control peptide (100 nM) for 1 hr and mitochondrial SIRT1 protein levels (A and B) and deacetylase activity (C) determined 48 hrs later (n = 4). In (D) PKCε activation was inhibited with the PKCε specific inhibitor ɛV1-2 (100 nM) during and following 45 min of oxygen and glucose deprivation (IPC) and the level of mitochondrial SIRT1 determined 48 hrs later (n = 3) in non-treated (Nt), control peptide and ɛV1-2 treated samples. Western blot quantitation is shown in (E). The effect of ɛV1-2 (100 nM) on IPC induced mitochondrial SIRT1 deacetylase activity is shown in (F) (n =4). Data are means ± SEM compared to control (Ctrl) TAT peptide treated cultures. * p < 0.05 increase from controls by Student’s t-test.