Figure 5. HSP90 is required for SIRT1 mitochondrial import.

Mitochondrial SIRT1 and PKCε protein levels were determined, 48 hrs following IPC exposure, in neuronal cultures treated with the HSP90 inhibitor, 17-AAG (100 nM) (A). Western blot quantitation is shown in (B and C; n = 3). The effects of 17-AAG treatment on whole cell SIRT1 and PKCε are shown in (D). Data are means ± SEM compared to vehicle (Veh) treated cultures. * p < 0.05 increase from controls by Student’s t-test.