Table 1. RSV A & B oligonucleotide primers and probes used in this study.
| S. No. | Primer Name | Sequence (5'–3') | Reference |
|---|---|---|---|
| 1 | Real-time RT-PCR for RSV | ||
| RSV Forward | GGCAAATATGGAAACATACGTGAA | US-CDC protocol (unpublished) | |
| RSV Reverse | TCTTTTTCTAGGACATTGTAYTGAACAG | ||
| RSV probe | FAM-CTGTGTATGTGGAGCCTTCGTGAAGCT-BHQ-1 | ||
| 2 | Conventional RT-PCR and Sequencing Primers for RSV-A | ||
| ExternalPCR | 30 | ||
| RSVA-G513-F | AGTGTTCAACTTTGTACCCTGC | ||
| RSVA-F131-R | CTGCACTGCATGTTGATTGAT | ||
| NestedPCR | |||
| RSVA-G606-F | AACCACCACCAAGCCCACAA | ||
| RSVA-F22-R | CAACTCCATTGTTATTTGCC | ||
| 3 | Conventional RT-PCR and Sequencing Primers for RSV-B | ||
| Forward primer BGF | GCAGCCATAATATTCATCATCTCT | 31 | |
| Reverse primer BGR | TGCCCCAGRTTTAATTTCGTTC | ||
| BGF Seq.1 | AGAGACCCAAAAACACYAGCCAA | ||
| BGR Seq.2 | ACAGGGAACGAAGTTGAACACTTCA |