Figure 4.

Recorded pictures taken with CCD camera system to capture bioluminescence in Neurospora crassa from a PDMS microfluidic device with gas perturbation system: (a) 1 h (O₂); (b) 24 h (O₂); (c) 33 h (CO₂; no light signal); and (d) 51 h (O₂). Representative conidia band is indicated. The growth rates were measured: ∼1.17 mm/h in O₂ gas; ∼0.38 mm/h in CO₂ gas. (e) Bioluminescence data from a Neurospora strain containing frq-luciferase reporter in the microfluidic device with a real-time gas perturbation system.