Table 5.

Percent post-thaw acrosome integrity of Sprague–Dawley rat sperm using various cooling rates and freezing extenders.

Cooling Rates	Fresh Sperm	TL- HEPES	m-KRB	SM	TRIS-R	TRIS-S	TES-R	TES-S
10 °C/min	76.2±2.9^b	23.3±3.7^a	26.3±3.6^a	22.2±3.6^a	26.5±4.4^a	29.0±4.6^a	25.0±5.0^a	27.3±3.4^a
40 °C/min	76.0±6.1^b	24.0±4.0^a	26.2±3.5^a	25.3±3.3^a	27.7±2.8^a	32.2±3.4^a	27.3±3.6^a	29.7±4.5^a
70 °C/min	76.3±5.7^b	23.3±3.7^a	26.8±4.2^a	21.2±2.2^a	24.5±3.1^a	25.5±4.8^a	26.8±3.2^a	26.2±4.5^a
100 °C/min	74.3±4.6^b	25.0±3.3^a	26.3±3.9^a	23.3±3.7^a	24.7±3.7^a	26.8±3.5^a	27.0±4.1^a	27.8±4.4^a
Significance	-	-	-	-	-	-	-

Values are mean percentages ± SEM (n = 6)

^a–b

Different superscripts within the same row denote significant differences (P < 0.05)

Differences between the within the same columns are not significant (P>0.05).