Table 1.
Figure | Beamline | Pixel size μm(v)×μm(h) |
Beam size μm(v) × μm(h) |
Dwell time (sec) |
Flux (photons/s) |
X-ray energy (keV) |
---|---|---|---|---|---|---|
1B, C | APS, 18-ID | 20 × 20 | 5 × 5 | 0.2 | 2×1013 | 10.0 |
1D | APS, 2-ID-D | 3 × 3 | 0.2 × 0.25 | 1.0 | 4×109 | 10.0 |
2A, B, 3A, C, S1, S2 |
APS, 2-ID-D | 0.3 × 0.3 | 0.2 × 0.25 | 1.0 | 4×109 | 10.0 |
3B | APS, 2-ID-D | 0.35 × 0.35 | 0.35 × 0.35 | 1.0 | 4×109 | 12.0 |
4A † | ALS, 10.3.2 | 6 × 6, 3 × 3 | 6 × 6, 3 × 3 | 0.5 | 3.5×108 | 11.5 |
4B, C, D † | APS, 18-ID | 5 × 5 | 5 × 5 | 0.05 | 2×1013 | 10.0 |
Tissue sections of 30 μm thickness were analyzed at ALS, 10.3.2 and APS 18-ID-D beamlines, while 10 μm thick sections were used for high resolution measurements at APS, 2-ID-D beamline.
Sections aged 16 weeks old were imaged at APS 18-ID-D (BioCAT) were used for analysis. All other sections used for analysis in Fig. 4 were imaged at ALS 10.3.2.