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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Aging Cell. 2013 Jul 8;12(5):823–832. doi: 10.1111/acel.12112

Table 1.

XRF imaging conditions

Figure Beamline Pixel size
μm(v)×μm(h)
Beam size
μm(v) × μm(h)
Dwell
time
(sec)
Flux
(photons/s)
X-ray
energy
(keV)
1B, C APS, 18-ID 20 × 20 5 × 5 0.2 2×1013 10.0
1D APS, 2-ID-D 3 × 3 0.2 × 0.25 1.0 4×109 10.0
2A, B, 3A, C,
S1, S2
APS, 2-ID-D 0.3 × 0.3 0.2 × 0.25 1.0 4×109 10.0
3B APS, 2-ID-D 0.35 × 0.35 0.35 × 0.35 1.0 4×109 12.0
4A ALS, 10.3.2 6 × 6, 3 × 3 6 × 6, 3 × 3 0.5 3.5×108 11.5
4B, C, D APS, 18-ID 5 × 5 5 × 5 0.05 2×1013 10.0
*

Tissue sections of 30 μm thickness were analyzed at ALS, 10.3.2 and APS 18-ID-D beamlines, while 10 μm thick sections were used for high resolution measurements at APS, 2-ID-D beamline.

Sections aged 16 weeks old were imaged at APS 18-ID-D (BioCAT) were used for analysis. All other sections used for analysis in Fig. 4 were imaged at ALS 10.3.2.