Table 3.
Results of the linear fits for S / Cu correlation (y = ax + b)
| Brain | a ([S]/[Cu] in μg/cm2) |
b | R2 | N | [Cu]* mM |
[S]/[Cu] molar ratio |
|
|---|---|---|---|---|---|---|---|
| Rat 1 | area 1 | 0.60 ± 0.01 | 1.40 ± 0.02 | 0.71 | 42 | 237.62 ± 81.8 | 1.19 ± 0.02 |
| area 2 | 0.53 ± 0.01 | 1.69 ± 0.03 | 0.65 | 30 | 203.00 ± 91.2 | 1.05 ± 0.02 | |
| Rat 2 | area 1 | 0.53 ± 0.00 | 1.79 ± 0.01 | 0.71 | 77 | 313.16 ± 113.3 | 1.05 ± 0.00 |
| Rat 3 | area 1 | 0.73 ± 0.01 | 1.60 ± 0.02 | 0.86 | 37 | 168.38 ± 62.9 | 1.45 ± 0.02 |
| area 2 | 0.52 ± 0.01 | 1.63 ± 0.02 | 0.70 | 36 | 240.77 ± 95.9 | 1.03 ± 0.02 | |
| area 3 | 0.54 ± 0.00 | 1.62 ± 0.01 | 0.96 | 51 | 341.48 ± 135.3 | 1.07 ± 0.00 | |
| Mouse 1 | area 1 | 0.59 ± 0.00 | 1.07 ± 0.00 | 0.95 | 190 | 368.24 ± 228.1 | 1.17 ± 0.00 |
| Mouse 2 | area 1 | 0.54 ± 0.00 | 1.26 ± 0.01 | 0.96 | 120 | 398.13 ± 173.1 | 1.07 ± 0.00 |
N – Number of pixels with high Cu content selected by cluster analysis of XRF images recorded on the brain tissue with single cell resolution.
*
Numbers are obtained with the assumption that intracellular compartments are about 1 micron in diameter and the signal detected in XRF pixel on 10 micron thick section comes from single compartment.