Amino acid alignment of GP2 MPER regions from type members of the five species of genus Ebolavirus (BDBV, Bundbugyo virus; TAFV, Thai Forest virus). Residues that are identical in at least four of the viruses are highlighted in gray (hydrophobic) or boxed (polar). For comparison, the MPER segments of FIV and HIV-1 gp41 are included. Z-MPER and S-MPER peptides correspond the sequences shown here for EBOV and SUDV, respectively. N- and C-termini of peptides were blocked with acetyl and amide groups, respectively.