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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Naunyn Schmiedebergs Arch Pharmacol. 2013 Jun 22;386(10):885–891. doi: 10.1007/s00210-013-0894-8

Figure 1.

Figure 1

Apparent expression of P2Y6 receptor in BSM using Alomone Labs antibody (APR-011). A). Lysates of mouse detrusor muscle (25 µg protein/lane) were immunoblotted with antibody APR-011. Lanes 1 and 2: P2Y6 antibody preabsorbed with buffer or with immunizing peptide. Lanes 3 and 4: detrusor muscle was lysed with non-denaturing co-immunoprecipitation lysis buffer and resolved under non-reducing (lane3) and reducing (lane 4) conditions by presence or absence of dithiothreitol in sample buffer. B) and C). Cryosections (5µm) of mouse bladders were labeled with antibodies to P2Y6 - green, Entpd2 to label the interstitial cells - red and Topro-3 to label nuclei - blue. Color merged panels are shown on the right. Scale bars = 10 µm.