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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Microbiol. 2013 Aug 14;89(6):1140–1153. doi: 10.1111/mmi.12337

Figure 2.

Figure 2

Expression and purification of recombinant BosR. SDS-PAGE analysis of whole-cell lysates of E. coli obtained before (lane 1) and after (lane 2) addition of IPTG indicated that expression of the His6-tagged BosR was induced by IPTG. SDS-PAGE analysis of the His6-tagged (lane 3) and the tag-free (lane 4) BosR indicated that these proteins were purified to apparent homogeneity. Sizes of protein markers were indicated in kDa.