Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Microbiol. 2013 Aug 14;89(6):1140–1153. doi: 10.1111/mmi.12337

Figure 5.

Figure 5

BosR recognizes the palindromic sequences near the ospAB and the ospD promoters. (A) Electrophoretic analysis of the fluorescent probes FPospAB, FPospD, FPRndAT, and FPRnd. For each probe, the 6-FAM-labeled single-stranded oligo (lane 1), the unlabeled single-stranded oligo (lane 2), and the double-stranded oligo (lane 3) were analyzed. (B) BosR binding curves to FPospAB (open or closed circle), FPospD (open or closed square), FPRndAT (open or closed triangle) and FPRnd (open or closed diamond) were generated using a FA-based assay. Data obtained using the His6-tagged BosR were indicated with closed symbols and solid lines, and data obtained using the tag-free BosR were indicated with open symbols and dashed lines.