BosR protects specific regions of B. burgdorferi genome from DNase I digestion. Fold of protection by BosR was determined by q PCR analysis for the following regions of B. burgdorferi genome: ospA (−330 ~ +20) (closed circle), ospA (+166 ~ +295) (open circle), ospD (−231 ~ +68) (closed square), rpoS (−277 ~ +60) (open triangle), bicA (−170 ~ +159) (closed diamond), bicA (−170 ~ −1) (open diamond), bosR (−183 ~ +10) (closed triangle). The ends of each targeted region are indicated by their distances to the start of the gene, with a negative value indicating a position upstream and a positive value indicating a position downstream.