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. 1971 May;21(5):888–892. doi: 10.1128/am.21.5.888-892.1971

Resistance to d-Cycloserine in the Tubercle Bacilli: Mutation Rate and Transport of Alanine in Parental Cells and Drug-Resistant Mutants

Hugo L David 1
PMCID: PMC377303  PMID: 4995732

Abstract

A single transport system was found to accumulate l- and d-alanine, glycine and d-serine in Mycobacterium tuberculosis. The results of inhibition experiments suggested that the antibiotics d-cycloserine and O-carbamyl-d-serine were also transported by the alanine-glycine-d-serine system. A d-cycloserine-resistant permease-competent (d-CSr/perm+) mutant and a d-cycloserine-resistant permease-defective (d-CSr/perm−) mutant were isolated. The d-CSr/perm− mutant was not found to be more resistant to the drug than was the d-CSr/perm+ mutant. The data were consistent with the conclusion that resistance to d-cycloserine in the tubercle bacilli is primarily due to mutations in the gene(s) controlling the enzyme d-alanyl-d-alanine synthetase. The mutation rate was calculated to be about 10−10 mutations per bacterium per generation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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