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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: J Am Chem Soc. 2013 Jun 26;135(27):9980–9983. doi: 10.1021/ja402927u

Figure 2.

Figure 2

Melting temperatures of 48G7 variants in the absence and presence of 1 mM hapten JWJ1: (A) single somatic mutations added to 48G7g and (B) single mutations added to 48G7b (binding mutant: G56HV/N77HK/D55LH). The three mutations that decrease the Tm of 48G7g the most are highlighted in red. Measurements were repeated twice for these mutants; error bars represent standard error.