Figure 3.

Close-up views of key interactions: (A) The somatic mutation N57^HD introduces hydrogen-bonding interactions with residues R50^H and K59^H. (B) The somatic mutation N77^HK results in the loss of two hydrogen bonds with residues A24^H and F27^H in the germline structure. The somatic mutation A79^HT enhances hydrophobic interactions with M34^H. (C) The hydrophobic linker of JWJ1 is surrounded by four key tyrosine residues (Y32^L, Y91^L, Y99^H, and Y100^H). Within this hydrophobic pocket lie H35^H and R96^L, which form an oxyan ion hole in which binds the phosphonate group. Mutation D55^LH removes hydrogen-bonding interactions with R46^L.