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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: J Immunol. 2013 Aug 12;191(6):3179–3185. doi: 10.4049/jimmunol.1301283

Figure 5. Analysis of b12HL B cell function in mixed chimeras with wild type cells.

Figure 5

Irradiated Rag1−/− mice were reconstituted with a mixture of b12HL and WT BM at a 1:1 ratio. At six weeks post transfer, reconstitution was assessed and immunization initiated. A, Analysis of peripheral blood taken at 6 weeks for the presence of Env-binding (b12HL) and non-binding B cells by gating on TCRβ− lymphocytes and staining for B220 and YU2 gp120 binding. B, CD93 density on B220+YU2− and B220+YU2+ cells. C,D, Serum IgM and IgG responses to Env immunization at various weeks post priming. Chimeric mice were immunized with 40 μg JRFL gp140 in Sigma Adjuvant and boosted three weeks later with antigen in saline. Data are from a total of eight mice as indicated carried out in one experiment.