Skip to main content
. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: J Immunol. 2013 Aug 12;191(6):3179–3185. doi: 10.4049/jimmunol.1301283

Figure 6. Antibody response of b12H mice to soluble Env trimers.

Figure 6

b12H and WT mice were challenged with soluble Env JRFL gp140 trimers and antibody responses measured using the JRFL gp140, YU2 gp120 or 2bodx_43 as the capture reagent in ELISA. The immunization protocol was as in Figure 3, with 4 mice/group. A, Ability of naive b12H B cells to bind to biotinylated YU2 gp120 and 2bodx_43. B,C, JRFL gp140-specific IgM and IgG response. D,E, IgG responses cross-reactive to YU2 gp140 or 2bodx_43. F, Neutralization of JRFL pseudotyped virus by immune sera from four indicated mice. Non-immunized b12HL sera were used as positive control. G,H, Analysis of IgG1 hybridomas from b12H mice. G, ELISA analysis of Env binding by selected hybridoma proteins to plate-bound JRFL gp140. H, Flow cytometry analysis of the binding of the indicated IgG1 hybridoma proteins to 293 cells transfected with full-length JRFL Env (cleaved, lower panels) or JRFL carrying a mutated furin cleavage site (uncleaved, upper panels).