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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Biol Psychiatry. 2013 Jun 14;74(10):10.1016/j.biopsych.2013.04.025. doi: 10.1016/j.biopsych.2013.04.025

Figure 3. Scopolamine administration increases spine density and maturation in PFC layer V pyramidal cells.

Figure 3

After whole-cell recordings slices were subjected to post hoc two-photon microscopy image of the neurobiotin-labeled layer V pyramidal cells. (a) Representative images are shown of high magnification Z-stack projections of distal and proximal segments of the layer V pyramidal cell apical tuft dendrites (Scale: 10 µm). (b) The density of spines was analyzed using automated software (MBF Bioscience, Neurolucida V10/Autospine) and the results are the mean ± SEM (12 cells from 5 rats for control and 14 cells from 6 rats for scopolamine). (c, d) Quantification of distal and proximal spine head diameter, and cumulative fraction curves; note the increase in population of large diameter, mushroom spines in the scopolamine group as compared to the saline controls *p < 0.05; **p < 0.01; Student’s t-test).