Fig. 7. Cav3.2 and BK channels co-localize in the same membrane area and belong to the same molecular complex.
(A–C) Confocal immunofluorescence images of an LNCaP cell overexpressing Cav3.2 GFP (green) stained with an anti-BK antibody (red). Staining is more pronounced on the plasma membrane for both channels and the overlay shows that there is a co-localization (yellow-orange areas) on plasma membrane areas. Scale bar: 10 µm. (D) Representation of both Cav3.2 and BK fluorescence intensities along the horizontal line shown in panel C. Inset: a scattergramme of BK fluorescence vs Cav3.2 fluorescence showing a correlation between both channels (Pearson's r = 0.77). (E) Western-blot of proteins immunoprecipitated by the anti-Cav3.2 antibody (anti-α1H) or the anti-BK antibody. Membranes were revealed with the anti-BK antibody and the anti-α1H (right panel) antibody. Bead lanes contain the beads used during the immunoprecipitation without the protein input.