Table 1.

Oligonucleotide primers used for establishment of duplex PCR.

Haemoprotozoan	Primer	Region amplified	Product size
B. bigemina [10]	Bg3 5′TAG TTG TAT TTC AGC CTC GCG 3′	Small subunit ribosomal RNA sequence of B. bigemina	689 bp
	Bg4 5′ AAC ATC CAA GCA GCT AHT TTA G 3′
T. evansi [11]	TR3 5′ GCG CGG ATT CTT TGC AGA CGA 3′	Repetitive nucleotide sequences of T. evansi	257 bp
	TR4 5′ TGC AGA CAC TGG AAT GTT ACT 3′