Table 2.
Levels of cytokines tested in the untreated and LPS-stimulated MKD patients' monocytes, both at 37°C and 40°C.
| Cytokine | Patients' monocytes | |||
|---|---|---|---|---|
| 37°C | 40°C | |||
| Untreated | LPS | Untreated | LPS | |
| IL-1β | 11.8 ± 10.5 | 1235 ± 146.2*** | 2 ± 0.5 | 494.3 ± 165.8* |
| IL-1ra | 283.8 ± 45.8 | 864.6 ± 117.4** | 267.7 ± 26.4 | 769.4 ± 47.1** |
| IL-2 | 79.9 ± 0.8 | 114.8 ± 3.3*** | 70.3 ± 2.1 | 107.3 ± 5*** |
| IL-4 | 4.1 ± 0.5 | 13.8 ± 0.6*** | 3.8 ± 0.5 | 12.8 ± 0.9*** |
| IL-5 | <2 | <2 | <2 | <2 |
| IL-6 | 106.4 ± 59.6 | 21756 ± 3518*** | 54.4 ± 23.3 | 19145 ± 2204** |
| IL-7 | <2 | <2 | <2 | <2 |
| IL-8 | 6047 ± 3068 | 22571 ± 16271 | 4852 ± 3581 | 34676 ± 28376 |
| IL-9 | 52.1 ± 5.2 | 197.3 ± 13.3*** | 42.7 ± 5.6 | 192.1 ± 8.6*** |
| IL-10 | 39.8 ± 1 | 181.9 ± 69.9 | 37.4 ± 0.9 | 161.2 ± 42 |
| IL-12(p70) | 44.1 ± 2.6 | 89.3 ± 11** | 38.2 ± 1.8 | 68.9 ± 4.1* |
| IL-13 | 4.4 ± 0.1 | 7.6 ± 1.5 | 3.6 ± 0.1 | 6.3 ± 0.2 |
| IL-15 | 162.2 ± 0.8 | 185.4 ± 5.3* | 153.3 ± 5 | 176.3 ± 3.2* |
| IL-17 | 266.4 ± 0.6 | 500.3 ± 11.4*** | 251.6 ± 7.4 | 495.6 ± 24.5*** |
| Eotaxin | 160.5 ± 2.9 | 216.5 ± 5** | 155.9 ± 2.8 | 205.9 ± 11.2** |
| FGF-basic | 160.6 ± 6.1 | 262.2 ± 2.2*** | 163.9 ± 4.4 | 251.1 ± 10.5*** |
| G-CSF | 50.3 ± 2.7 | 1235 ± 554.8 | 40.9 ± 2.5 | 611.1 ± 117.5 |
| GM-CSF | 1417 ± 46.6 | 1718 ± 102.1 | 1398 ± 17.3 | 1462 ± 46.9 |
| IFN-γ | 103.3 ± 7.7 | 324.3 ± 10.3*** | 128.2 ± 2.1 | 308.6 ± 15.7*** |
| IP-10 | 78.4 ± 4.1 | 146.2 ± 5.8*** | 91.7 ± 10.8 | 131.3 ± 3.2* |
| MCP-1 | 519.4 ± 239.2 | 430 ± 159.7 | 89.7 ± 32.8 | 522.7 ± 224.9 |
| MIP-1α | 28.5 ± 22.3 | 8980 ± 1899** | 19.9 ± 12.9 | 8234 ± 963.1** |
| PDGF-BB | 80 ± 7.2 | 129.6 ± 3*** | 66 ± 3.5 | 112.7 ± 2.7*** |
| MIP-1β | 336.1 ± 127.4 | 33350 ± 3709 | 343.2 ± 82.7 | 42701 ± 18032 |
| RANTES | 271.9 ± 120.3 | 474.5 ± 71.9 | 191.7 ± 70.3 | 401 ± 50.2 |
| TNF-α | 66.7 ± 1.9 | 3613 ± 1076 | 57.5 ± 6.1 | 3025 ± 1146 |
| VEGF | 77.9 ± 6 | 116.3 ± 11.5* | 68.8 ± 2.5 | 106.4 ± 5.8* |
| IL-1α | 29.6 ± 26 | 228.2 ± 50.7 | 4.5 ± 1.6 | 183.6 ± 70.2 |
| IL-2Rα | 101 ± 7 | 98.9 ± 19.5 | 103 ± 15.9 | 105.1 ± 14.3 |
| IL-3 | 726.6 ± 182.8 | 1244 ± 266.9 | 945.6 ± 113.2 | 907 ± 246.8 |
| IL-12(p40) | 3081 ± 716.6 | 6938 ± 2344 | 2754 ± 282.1 | 3811 ± 1151 |
| IL-16 | 128.2 ± 47 | 94.6 ± 14.4 | 118.3 ± 14.1 | 130.9 ± 32.9 |
| IL-18 | 8.3 ± 3.6 | 12.4 ± 0.9 | 5.4 ± 2.3 | 8.4 ± 5.8 |
| CTACK | 15.6 ± 11.9 | 5.3 ± 5.1 | 24.7 ± 24.5 | 25.8 ± 21.3 |
| GRO-α | 2006 ± 903.3 | 7845 ± 2004 | 272.1 ± 142.3 | 5195 ± 996.4 |
| HGF | 85.4 ± 3.8 | 54.2 ± 8.5 | 56.2 ± 8.5 | 77.6 ± 10.2 |
| IFN-α2 | 97.8 ± 18.9 | 78.6 ± 5.7 | 69.8 ± 15.5 | 104.2 ± 15.7 |
| LIF | 330.2 ± 37.5 | 302.9 ± 12.3 | 284.2 ± 17.3 | 288.3 ± 42.1 |
| MCP-3 | 455.8 ± 130.6 | 298.4 ± 32 | 118.2 ± 64.6 | 82.5 ± 71.6 |
| M-CSF | 127.2 ± 27.3 | 169.5 ± 31 | 117.8 ± 7 | 144.7 ± 10.2 |
| MIF | 1681 ± 465.5 | 1034 ± 190.1 | 1644 ± 191.1 | 1913 ± 281.3 |
| MIG | 45.6 ± 0.5 | 37 ± 1.7 | 36.9 ± 15.3 | 42.2 ± 3.4 |
| β-NGF | <2 | <2 | <2 | <2 |
| SCF | 33.5 ± 16.2 | <1 | <1 | 5.9 ± 4.9 |
| SCGF-β | 4375 ± 2343 | 969.5 ± 930.9 | 1683 ± 1645 | 1320 ± 1282 |
| SDF-1α | 209.4 ± 43.2 | 425.5 ± 90.8 | 160.4 ± 19.9 | 342.9 ± 72.3 |
| TNF-β | 41.9 ± 2.8 | 46.7 ± 7.2 | 42.1 ± 11.2 | 46.6 ± 11.7 |
| TRAIL | 31.6 ± 15.8 | 35.8 ± 6.5 | 16.3 ± 15.3 | 11.4 ± 10.4 |
Values are expressed as mean ± SEM, in pg/mL; *P < 0.05, **P < 0.01, and ***P < 0.001 versus the untreated groups tested at the same temperature: one-way ANOVA test followed by Bonferroni's multiple comparison test.