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Antioxidant mechanism of the PCS extract on hepatocytes. HepG2 cells were treated with (200 μg/mL) of the PCS extract for 24 h, and 1 mM H2O2 was added for the last 3 h. (a)–(d) The mRNA levels of antioxidation-related genes were analyzed by qRT-PCR. (e) Protein levels of antioxidation-related enzymes were analyzed using western blotting. (f) SOD activity. (g) GPX activity. *P < 0.05 versus (−)/H2O2. CON, treatment with nothing. Resveratrol (Res, 50 μM) was used as a positive control.
