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. Author manuscript; available in PMC: 2014 Apr 23.
Published in final edited form as: ACS Nano. 2013 Apr 10;7(4):3036–3044. doi: 10.1021/nn4006544

Figure 4. Immunization of mice with NP-P22 induces significant NP-specific CD8+ T cell responses following influenza infection.

Figure 4

a) Survival of mice infected with 50× LD50 of PR8 influenza 26 days after completion of immunization and re-challenged with 50× LD50 of X31 influenza 20 days later. CD8+ T cell-depleted mice are identified with addition of TIB210 (+TIB210, green and blue traces). Survival curves show average survival of 8-10 mice per group. b) Flow cytometry analysis of cells isolated from BALF to determine the percent of NP-specific CD8+ T cells from mice immunized with NP-P22 (red), P22 (green), or PBS (blue). Bar graph (left) shows average percentage of tetramer+ cells from 5 mice per group and a representative histogram (right) shows the number of cells stained with NP-specific tetramer.