Figure 2.

GR7-short hairpin RNA (shRNA) injection in the nucleus accumbens increased ethanol intake and ethanol preference in a two-bottle choice drinking paradigm. (a) Ethanol consumption and (b) ethanol preference displayed by GR7-scr and GR7-shRNA in the two-bottle free-choice paradigm test were plotted over increasing concentrations of ethanol. (a) GR7-shRNA rats consumed more ethanol than GR7-scr rats and pooled data are shown. (b) Ethanol preference was indicated as a ratio of ethanol volume to total fluid consumed. (c) Total fluid intake at the dose indicated. Data are presented as the mean±SEM. ^*p<0.05, the difference between the indicated group (two-way analysis of variance, Bonferroni's post hoc test).