Figure 1.
AQP9 immunostaining in the proximal cauda epididymidis of an adult rat (A), a 4-week-old rat (C), and a 3-week-old rat (E). A bright staining was detected in the apical membrane of principal cells at all time points. The staining reached maximal intensity, comparable to adult levels, between the third and fourth postnatal weeks. (B, D and F) Pre-incubation of the antibody with the AQP9 peptide completely abolished the staining at all time points. B, control; D, 4-week-old; F, 3-week-old epididymis. Bars=25 μm.