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. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: Brain Res Mol Brain Res. 2002 Nov 15;107(2):97–107. doi: 10.1016/s0169-328x(02)00444-8

Figure 3. Analysis of steady-state levels of mRNA for σR1 and GAPDH in retina of control (C) and diabetic (D) mice.

Figure 3

Total RNA was isolated from neural retinas of mice at 3, 6, 12 weeks post-onset of diabetes and in age-matched control mice. Primers specific for mouse σR1 and mouse GAPDH mRNA were used for RT-PCR with appropriate PCR cycle numbers so that the product formation was within the linear range (24 cycles for σR1 and 12 cycles for GAPDH). (A) Representative Southern hybridization signal. (B) The relative band intensity (σR1/GAPDH) in diabetic retina relative to control retina. The σR1/GAPDH ratio in control retina was taken as 1.