Cryosections of mouse retina from control age-matched to diabetic mice (2, 6, 12 weeks post-onset of diabetes) were probed with an antisense digoxigenin-labeled σR1 riboprobe. The analysis showed a positive reaction (deep purple stain) in the retinal ganglion cells (arrow). A positive reaction was observed in the ganglion cells of diabetic mice at all ages examined. (A) 2 week control, (B) 2 week diabetic, (C) 6 week control, (D) 6 week diabetic, (E)12 week control, (F) 12 week diabetic. gcl = ganglion cell layer, inl = inner nuclear layer. (Magnification: 400X).