FIG. 1.

Odd expression during lymph gland development. (a) The odd transcript levels in wild-type (wt) and ush^vx22/r24 trans-heterozygous (ush) lymph glands were determined using qRT-PCR. The Rpl32 transcript served as a reference. The relative level of odd expression in ush^vx22/r24 trans-heterozygous lymph glands was 0.62 6 ± 0.04 of wild-type (P < 0.0007). (b–d′) Odd expression was elevated in prohemocytes and downregulated in plasmatocytes. (b) Odd expression in a late embryonic lymph gland (elg). (c) Early-third larval instar lymph gland (72 h after egg laying) showing Odd expression in the medullary zone (MZ) and lack of expression in cortical zone plasmatocytes (PM). Plasmatocytes were identified using the specific marker Nimrod (P1). (c’) Inset displays the same lymph gland showing only odd expression. (d) Late-third larval instar lymph gland (144 hr after egg laying) showing Odd expression in green and plasmatocytes in red. (d’) Inset displays the same lymph gland showing only odd expression. (e–f″) Odd was downregulated in ush mutant lymph glands. Wild-type (wt) and ush^{vx22/ r24} trans-heterozygotes (vx22/r24) lymph glands from late-third instar lavae (144 h after egg laying). (e) wt and (f) vx22/r24 at ×0 magnification. (e’) wt and (f’) vx22/r24 show lymph glands photographed using bright field and fluorescence (merged) at ×40 magnification. (e”) wt and (f”) vx22/r24 show only fluorescence at ×40 magnification. Arrows mark pericardial cells (pc). Abbreviations: odd, odd-skipped; qRT-PCR, Quantitative Real-Time RT-PCR; ush, u-shaped. Scale bars: Panel b, 10 µm; Panels c–f″, 50 µm.