FIG. 3.

Plasmatocyte and lamellocyte differentiation increases with loss of Odd function. (a–c) Plasmatocyte differentiation increased in odd heterozygous (odd/+ lymph glands. Lymph glands were from late-third instar larvae (144 h after egg laying). Differentiation was monitored using the plasmatocyte specific marker, P1. (a) wild-type (wt). (b) odd/+ lymph gland. (c) Plasmatocyte production as a percentage of total lymph gland cells was 30 ± 17 in wt and 68 ± 19 in odd/+ lymph glands (P < 0.007). (d–f) Plasmatocyte differentiation was reduced in domeGal4 driven UAS-odd (dome> odd) lymph glands. Plasmatocyte differentiation was monitored using the specific marker, P1. Lymph glands were from mid-third instar larvae raised at 18°C (190 h, which is equivalent to 96 h after egg laying). (d) domeGal4 heterozygous control (dome/+ and (e) dome> odd lymph gland. (f) Plasmatocyte production as a percentage of total lymph gland cells was 60 ± 17 in dome/+ controls (+ and 30 ± 25 in dome> odd (Odd^GOF) lymph glands (P < 0.0001). (g–i) Lamellocyte differentiation increased in dome> odd^RNAi lymph glands. Lamellocyte differentiation was monitored using the specific marker, L1. Lymph glands were from mid-third instar larvae (96 h after egg laying). (g) dome/+ control and (h) dome> odd^RNAi lymph gland. (i) The average number of lamellocytes was 1 ± 0.8 in dome/+ controls (+ and 15 ± 5 in dome> odd^RNAi (odd^RNAi) lymph glands (P < 0.0001). Abbreviations: dome, domeless; odd, odd skipped. Scale bars: 100 µm.