Lamellocyte differentiation does not increase in odd/ush trans-heterozgyotes. Lamellocytes were identified using the specific marker, L1, and are rarely observed in (a) odd heterozygotes (odd/+, (b) ush heterozygotes (ush/+, and (c) odd/ush trans-heterozygotes (odd/ush). The number of lamellocytes was dramatically increased in (d) ushvx22/r24 trans-heterozygotes (vx22/r24). Lymph glands were counterstained with Dapi. Lymph glands were from late-third instar larvae (144 h after egg laying). Abbreviations: odd, odd-skipped; ush, u-shaped. Scale bars: 100 µm.