Confocal images of live brain slices stained with 100 nM ASP+. A - Control staining of hippocampus CA3 zone observed with 10× objective. B. The same CA3 zone with 40× oil objective. Stratum radiatum (with fluorescent astrocytes) and pyramidal cells layer with low fluorescent neurons can be seen (see also Web content Fig 2 for 3D image of this zone).
C- Low magnification (10 × objective) of VT midbrain slice, showing overall layout of fluorescent cells in that zone. D - Fluorescent neurons and blood vessels in VTA at higher magnification (40× oil objective), while some of neurons have fluorescence lower, than background (see also Web content Fig 2 for 3D image of this zone).