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. 2013 Aug 26;110(37):14990–14994. doi: 10.1073/pnas.1305999110

Fig. 4.

Fig. 4.

(A) Gonadal WAT was extracted from 20-wk-old mice (19 GNB3/+ male, 12 +/+ male, 14 GNB3/+ female, and 11 +/+ female mice) and weighed to calculate WAT percent. GNB3/+ WAT percent is significantly greater than +/+ WAT percent in *females (P = 0.0122) and **males (P < 0.0001; Student t test). (B) GNB3 is highly expressed in GNB3/+ brains. Mean Ct values from qRT-PCR of human GNB3, mouse Gnb3, human MLF2, and mouse Mlf2 transcripts in whole brains from eight 8-wk-old male mice are plotted. Data from GNB3/+ and +/+ littermates are shown as solid bars; data from ΔGNB3/+ and +/+ littermates are shown as hatched bars. Lower Ct values correspond to greater gene expression; the absence of Ct data indicates no detection of transcript. All qRT-PCR experiments were performed in triplicate with an internal mouse β-actin (Actb) control. SEM for 40 Actb triplicates was ±0.05 Ct; maximum SEM for other transcripts was ±0.31 Ct.