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. 2013 Aug 26;110(37):14972–14977. doi: 10.1073/pnas.1220884110

Fig. 3.

Fig. 3.

Ci-Slc26aα is essential for lumen expansion, but not for apical/luminal membrane specification and biogenesis or lumen connection. (A–D′) Notochord in control (A–D) and Ci-Slc26aαMO1–injected embryos (A′–D′). (E) Narrow tube in Ci-Slc26aαMO1–injected notochord. (F–I) Sensory vesicle in control embryo (F; Ci-Slc26aαMO2C–injected) and embryos injected with Ci-Slc26aαMO1 (G) or Ci-Slc26aαMO2 (H and I). (J and J′) Ci-Slc26aα expression rescued the lumen formation defect. (K and K′) Ci-Caveolin 1-mCherry was localized at the apical/luminal domain in control MO-injected embryos. (L–M′) Apical/luminal membrane, marked by Ci-Cav-1-mCherry (L and L′) or Ci-Slc26aα E419K-mCherry (M and M′), developed in Ci-Slc26aαMO2–injected notochord cells. The white dashed line in J outlines the apical/luminal membranes of cells next to Ci-Slc26aα-mCherry–expressing cells, the white arrowhead indicates the lumen, the yellow arrowhead indicates the absence of lumen, and the green arrowhead indicates the presence of apical/luminal membrane. mCh, mCherry.