Fig. 3.

Candidate genes have PC2_COL mutant phenotypes; PHO1/At3g23430 and RSA1/At4g28410 show G × E interaction for KNO₃, ABA, and IAA conditions. (A) PC2_COL values of mutant alleles for six candidate genes (n = 22–31) grown in KNO₃ media plates, compared with wild-type Col-0 (n = 36). (B) Quantitative PCR on the rsa1-1 gain-of-function mutant allele (n = 3). (C–E) Reaction norms testing G × E interactions for IAA, ABA, CK, KNO₃, and NH₄Cl in pho1-2, rsa1-1 gain-, and rsa1-2 loss- of function mutants, respectively (n ≥ 11). Only significant interactions (KNO₃, ABA, and IAA) are shown: *P < 0.05, **P < 0.005, ***P < 0.0005. Error bars: SE.