Fig. 4.

Antibody increases the motility of human bone marrow CD34⁺ cells. (A) CD34⁺ cells were incubated with PBS or antibodies for 16 h in transwell chambers. The values are the mean +/− SE of three independent experiments. *P < 0.05 versus basal values. (Lower) Fluorescent dye-labeled cells from the lower chamber by fluorescent microscopy. (B) CD34⁺ cells were cultured in methylcellulose agar with antibody or PBS for 6 d. Images are from bright-field microscopy analysis. (C) CD34⁺ cells were incubated with PBS or antibodies for 10 min at 37 °C and analyzed by Western blots with anti–p-AKT, AKT, p-ERK, and ERK antibodies.